ABSTRACT
Objective To discuss the efficacy of six ingredient rehmannia pill combined with metformin in treatment of type 2 diabetes with Qi-Yin deficiency.Methods 120 cases with type 2 diabetes were selected.They were divided into two groups randomly.The observation group (60 cases) took six ingredient rehmannia pill combined with metformin.The control group (60 cases) took metformin.The efficacy of interlocking intramedullary nail and fracture contusion capsule in treatment of open tibial fracutre was evaluated by fasting blood glucose,postprandial 2 h blood glucose,total symptom scores before and after treatment,adverse reaction during treatment.Results The effective rate of observation group was significantly higher than that of the control group (P ＜ 0.05).Before treatment,fasting blood glucose and postprandial 2 h blood glucose level had no significant difference between two groups.After treatment,the blood glucose of two groups were decreased (P ＜ 0.05).The fasting blood glucose and blood glucose 2 h after meal of observation group were lower than that of the control group (P ＜ 0.05).Before treatment,total symptom scores had no significant difference.After treatment,the two groups of the total symptom scores were decreased significantly (P ＜ 0.05),and the observation group the total symptom score lower than that of the control group (P ＜ 0.05).During the treatment,the control group of 1 cases of hypoglycemia,the observation group of 1 cases of hypoglycemia,1 cases of mild nausea.The adverse reactions of two groups had no significant difference.Conclusion Six ingredient rehmannia pill combined with metformin had good effect on type 2 diabetes.It could significantly improve the symptoms of diabetes diagnosed by traditional Chinese medicine.The hypoglycemic effect was obvious with high drugy safety.It was worthy of clinical application.
ABSTRACT
Objective To explore the biological characteristics and biofilm formation of a strain of Klebsiella pneumoniae isolated from the stool of a patient with clinical inflammatory bowel disease .Methods The TH6 strain was identified as Klebsiella pneumoniae by 16S RNA, MALDI-TOF and automatic biochemical instrument .The morphology, growth rate, biofilm formation, capsid virulence factors and other biological characteristics were analyzed .Results and Conclusion The TH6 strain was short rod-shaped with a capsule under the transmission electron microscope , and was of K57 type according to the capsule serotype test .K.pneumonia TH6 grew rapidly under aerobic and anaerobic conditions , almost at twice the rate of standard strain ATCC2146, and reached the logarithmic growth phase at 2 h.The strain had multi-drug resistance.Biofilm formation was observed under a confocal laser scanning microscope (CLSM) at 4, 8, 12, 24, 48, 72, 96 and 120 h and the biofilm was formed at 72 h.The ability of fast growth , biofilm formation and other characteristics of K.pneumonia TH6 showed that this strain has a strong ability to adapt to the new environment is difficult to cure after colonization in vivo.
ABSTRACT
Objective To discuss the efficacy of six ingredient rehmannia pill combined with metformin in treatment of type 2 diabetes with Qi-Yin deficiency.Methods 120 cases with type 2 diabetes were selected.They were divided into two groups randomly.The observation group (60 cases) took six ingredient rehmannia pill combined with metformin.The control group (60 cases) took metformin.The efficacy of interlocking intramedullary nail and fracture contusion capsule in treatment of open tibial fracutre was evaluated by fasting blood glucose,postprandial 2 h blood glucose,total symptom scores before and after treatment,adverse reaction during treatment.Results The effective rate of observation group was significantly higher than that of the control group (P ＜ 0.05).Before treatment,fasting blood glucose and postprandial 2 h blood glucose level had no significant difference between two groups.After treatment,the blood glucose of two groups were decreased (P ＜ 0.05).The fasting blood glucose and blood glucose 2 h after meal of observation group were lower than that of the control group (P ＜ 0.05).Before treatment,total symptom scores had no significant difference.After treatment,the two groups of the total symptom scores were decreased significantly (P ＜ 0.05),and the observation group the total symptom score lower than that of the control group (P ＜ 0.05).During the treatment,the control group of 1 cases of hypoglycemia,the observation group of 1 cases of hypoglycemia,1 cases of mild nausea.The adverse reactions of two groups had no significant difference.Conclusion Six ingredient rehmannia pill combined with metformin had good effect on type 2 diabetes.It could significantly improve the symptoms of diabetes diagnosed by traditional Chinese medicine.The hypoglycemic effect was obvious with high drugy safety.It was worthy of clinical application.
ABSTRACT
<p><b>OBJECTIVE</b>To construct a RNA interfering plasmid targeting rat Bcl-2/E1B-19K-interacting protein 3 (BNIP3) and assess its effect on exogenous BNIP3 expression in HEK293 cells.</p><p><b>METHODS</b>The miRNA sequences were designed using Invitrogen BLOCK-iT RNAi Designer and synthesized into double-strand oligonucleotides, which were cloned into the pcDNATM6.2-GW/EmGFP-miR vector, followed by transformation of the product into competent Top10 E. coli cells. After expansion of the transformed bacteria, the plasmid was extracted and sequenced before its co-transfection with pEGFP-C3- rBNIP3 plasmid into HEK293 cells. The interference effect of the constructed plasmid on BNIP3 mRNA and protein expression were detected by real-time PCR and Western blotting.</p><p><b>RESULTS</b>The sequencing result indicated that the interfering plasmid targeting rat BNIP3 was constructed correctly. After transfection into HEK293 cells, the interfering plasmid significantly inhibited exogenous BNIP3 mRNA and protein expressions.</p><p><b>CONCLUSION</b>The RNA interfering plasmid targeting rat BNIP3 has been constructed successfully, which provides a useful tool for studying the function of BNIP3.</p>
Subject(s)
Animals , Humans , Rats , Genetic Vectors , HEK293 Cells , Membrane Proteins , Genetics , Mitochondrial Proteins , Plasmids , Proto-Oncogene Proteins , Genetics , RNA, Messenger , Genetics , RNA, Small Interfering , Genetics , TransfectionABSTRACT
<p><b>AIM</b>To study the protection mechanisms of K(ATP) channels on hippocampal CA1 neurons during chronic severe hypoxia.</p><p><b>METHODS</b>p53 expression, DNA fraction, and cell apoptosis were examined in cultured hippocampal neurons in control group, hypoxia group, hypoxia group treated with K(ATP) channels antagonist and hypoxia group treated with K(ATP) channels agonist.</p><p><b>RESULTS</b>In the group of a 12 h long exposure to oxygen concentration of 0%, diazoxide (100 micromol/L), the K(ATP) channels agonist, reduced p53 expression and the hypoxia-induced apoptosis. In contrast, tolbutamide (100 micromol/L), the K(ATP) channels antagonist, significantly rose p53 expression and the hypoxia-induced apoptosis, which could be reversed by p53 inhibitor TSA.</p><p><b>CONCLUSION</b>K(ATP) channels protect hippocampal neurons against chronic severe hypoxia by suppressing p53 expression.</p>